FLUORESCENCE ANISOTROPY ASSAYS FOR HIGH THROUGHPUT SCREENING OF COMPOUNDS BINDING TO LIPID II, PBP1B, FTSW AND MURJ

Fluorescence anisotropy assays for high throughput screening of compounds binding to lipid II, PBP1b, FtsW and MurJ

Abstract Lipid II precursor Showman® 34" x 36" x 3/4" Teal and its processing by a flippase and peptidoglycan polymerases are considered key hot spot targets for antibiotics.We have developed a fluorescent anisotropy (FA) assay using a unique and versatile probe (fluorescent lipid II) and monitored direct binding between lipid II and interacting p

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Antimicrobial peptides: a new class of antimalarial drugs?

A range of antimicrobial peptides (AMP) exhibit activity on malaria parasites, Plasmodium spp, in their blood or mosquito stages, or both.These peptides include Brakes a diverse array of both natural and synthetic molecules varying greatly in size, charge, hydrophobicity and secondary structure features.Along with an overview of relevant literature

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Meta-analysis of Molteno glaucoma implants and Ahmed glaucoma valves: insights into efficacy and safety for complex glaucoma

BackgroundGlaucoma is a leading cause of irreversible blindness globally and for decades, Molteno and Ahmed glaucoma implants, operating on different mechanisms, have been used to treat complicated glaucoma with varying success.ObjectiveTo assess the safety and efficacy of the Molteno glaucoma implant (MGI) versus the Ahmed glaucoma valve (AGV) in

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