Fluorescence anisotropy assays for high throughput screening of compounds binding to lipid II, PBP1b, FtsW and MurJ
Abstract Lipid II precursor Showman® 34" x 36" x 3/4" Teal and its processing by a flippase and peptidoglycan polymerases are considered key hot spot targets for antibiotics.We have developed a fluorescent anisotropy (FA) assay using a unique and versatile probe (fluorescent lipid II) and monitored direct binding between lipid II and interacting p